FastQCFastQC Report
Wed 29 Oct 2014
uncut_2_CTGAAGCT-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_2_CTGAAGCT-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4205128
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3057027.269743037548442No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123140.29283294111380204No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98060.23319147478982802No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83750.1991615950810534No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75630.17985183804155308No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.14843781211891768No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.13607195785716866No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.12644085982638342No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49290.11721403010800148No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.1038731757986915No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.10339756601939346No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.10315976112974445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCGC2500.030.6027073
ATATGGA21950.023.7981451
ATATCTC14950.021.2357461
GATCCGG90550.018.798491
ATCTCGA34150.017.8676821
TATGGAT30900.017.6237582
TATCTCG18200.017.3100472
TCTCGAG37650.016.8548832
ACCCGGC2301.8189894E-1216.63272
ATCCGGC110100.015.57432652
ATAAAAA25700.015.4982192
TGCGCGC3550.015.2126153
TCGAGGG44850.014.7501184
CTCGAGG44150.014.4746683
ACACGTC4200.014.46350213
AATAAAA26850.014.33974551
CGAGGGC64850.014.0963171
CGTGGGC5700.013.82556251
CCCGGCG2957.2759576E-1213.7300283
ACCGGCG6750.013.6678773
Produced by FastQC (version 0.11.2)