FastQCFastQC Report
Wed 29 Oct 2014
uncut_1_old_TCTCGCGC-CAGGACGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_1_old_TCTCGCGC-CAGGACGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10802184
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256663423.76032476395514No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1019100.9434203305553766No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA716390.663189962326137No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214110.19820991755000655No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184740.17102097131468968No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA159050.14723874357259606No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148950.1378887824906519No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144190.13348226617876532No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136550.1264096223504432No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135200.12515987507711404No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124600.11534704463467757No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT120220.11129230903676515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG118740.10992221572970798TruSeq Adapter, Index 8 (97% over 36bp)
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115560.10697836659697706No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112130.10380308278399998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT21150.032.1273537
GCGCATC21550.031.84409138
CTCGCGC21650.031.48944935
TCGTATG22700.030.72742845
TCGCGCA23600.028.88756636
TCTCGCG23850.028.7734434
CTCGTAT26350.026.3005344
TCTCGTA27550.025.07328243
ACTCTCG28750.023.94748332
ATATGGA40850.023.8085771
GTCACTC30150.023.208329
CGCATCT31100.022.93375639
CATCTCG30600.022.72026341
ATCTCGT31900.021.79445842
AGTCACT33300.021.08048628
CAGTCAC33750.020.99931127
CTCTCGC34500.020.28250733
TGGGCGC3050.019.9209753
TCCAGTC36600.019.36420625
CCAGTCA36550.019.32914226
Produced by FastQC (version 0.11.2)