FastQCFastQC Report
Wed 29 Oct 2014
uncut_1_CTGAAGCT-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_1_CTGAAGCT-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3532397
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1867765.287514398862869No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84200.23836505353163873No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54980.15564501951507717No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51820.1466992526604456No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37450.10601866098289632No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.10375390988045793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC2000.025.8787293
ATATGGA19250.019.997821
GATCCGG62450.019.357941
TATCTCG11200.018.0832182
GTCGCGC750.001109664118.0028482
TATGGAT24600.016.3745422
CTGCGCG2750.016.3662282
ATCCGGC76700.015.8434452
TGGGCGC1855.6206773E-915.8130883
ATATCTC12750.015.7143751
ATCTCGA22850.014.6797061
TCGCGCC950.00537379514.2125743
TCCGGCG87800.013.8402623
TCGGCGC2306.672053E-913.6976263
TGGCGCG2154.071626E-813.6066113
CGAGGGC40000.013.50731
AATAAAA19500.013.50731
CCGGCGC98900.013.3333814
CGGCGCG101900.012.8745875
GTGGGCG2309.836367E-812.7194032
Produced by FastQC (version 0.11.2)