FastQCFastQC Report
Wed 29 Oct 2014
uncut_1_CTGAAGCT-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameuncut_1_CTGAAGCT-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5603668
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1777943.1728146635382397No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121930.21758962165495888No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80090.1429242417645014No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75020.1338765965435497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC2550.022.9442183
ATATGGA30400.019.3286191
GATCCGG84450.018.3143271
ATCTCGA35100.015.84251
TATGGAT39100.015.7694932
TGGCGCG3450.015.0019873
TCTCGAG38250.014.8844582
CTGCGCG3800.014.8047252
TCGAGGG41800.014.7503624
ATCCGGC106850.014.7423742
CGAGGGC63000.014.6513351
TATCTCG19050.014.52961352
ATATCTC19850.013.7233061
CCCCCCC1000.00755544113.50010144
CTCGAGG43150.013.3505523
GTGGGCG3051.2732926E-1113.2805672
GAGGGCG71200.013.2111382
AGGGCGC72400.012.89880853
GGATCCG48400.012.8784275
GGGCGCG76000.012.8204344
Produced by FastQC (version 0.11.2)