FastQCFastQC Report
Wed 29 Oct 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602711
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230247.018124752998831No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59100.12840258708400332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG1302.6268026E-819.13133241
TATCTCG13050.019.0724722
ATATCTC13350.018.488371
GTCGCGC952.8963608E-416.5222422
CGTGTGC1907.841663E-915.42032923
GACCGAC1907.923518E-915.40821437
ATATGGA25900.014.987651
TATGGAT26700.014.1928622
CGTAGGC2153.8280632E-813.67101542
TCGCGCC1150.00123455113.6483643
TGGATCC30500.013.5267554
TGGGCGC1200.001701455113.0796813
AATAAAA48450.012.7821081
GGATCCG32300.012.7729425
ATGGATC31400.012.1394673
CAGTCAC12100.012.07548327
CACACGT15450.011.90776112
GACGTGT2706.1229E-811.68759321
ACACGTC15900.011.147428513
ACGTCTG15950.011.11236215
Produced by FastQC (version 0.11.2)