FastQCFastQC Report
Wed 29 Oct 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4276114
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2333005.4558882200053604No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48070.11241515076539119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG13800.019.8404882
ATATCTC14000.019.2506331
GTCGCGC1102.3148023E-618.362062
ATATGGA25650.016.373671
TATGGAT27200.015.759272
TGGGCGC1351.6228614E-514.9611553
TGGATCC33500.013.59520154
GGATCCG33500.013.3924075
GTGGGCG1709.6686945E-613.2014832
AATAAAA35200.012.8884461
GATCCGG50750.012.3912121
TCGAGGG29300.012.251434
ATGGATC36600.011.9566593
TCTCGAG28150.011.7992652
ATCTCGA26850.011.794151
TATCCGG1953.9214105E-511.5174741
CTCGCGC2757.749259E-811.49075335
CTCGAGG29850.011.3524333
ATAAAAA45900.010.9034462
TATACGA3451.625449E-810.4042313
Produced by FastQC (version 0.11.2)