FastQCFastQC Report
Wed 29 Oct 2014
5hr_8_TCCGGAGA-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_8_TCCGGAGA-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958476
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315291.6098742083129942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2050.027.43787212
ACACGTC2150.027.2081613
ACTCCGG2500.023.40021132
CTCGTAT2500.022.50020444
GCACACG2650.021.22552311
CACGTCT2650.021.22552314
TCGTATG2700.020.83352345
ACGTCTG2850.020.52545415
CACTCCG3050.018.4427931
CTCCAGT3300.018.40878924
GTCACTC3150.017.85730629
TATCTCG4550.017.8037222
AATCTCG3500.016.07157341
TCTCGTA3650.016.02754243
ATCTCGT3700.015.81095542
ATATCTC4950.015.4610011
AGTCACT4300.013.60477528
GAGCACA4800.013.593189
AGAGCAC4400.013.2948968
GTCTGAA4800.013.1244517
Produced by FastQC (version 0.11.2)