FastQCFastQC Report
Wed 29 Oct 2014
5hr_8_TCCGGAGA-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_8_TCCGGAGA-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536710
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141400.920147588028971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1800.032.49772311
ACACGTC1900.030.78731313
TCGTATG1900.029.60607545
CACACGT2050.028.53458412
CTCGTAT2050.027.43977744
ACTCCGG2150.027.2100532
ACGTCTG2450.023.87587515
AATCTCG2700.023.33397541
TCTCGTA2800.021.69702343
CACTCCG2900.020.9488531
GTCACTC2700.020.83322729
CACGTCT2900.020.17114
ATCTCGT3500.018.6433742
CTCCAGT3500.017.35592524
GTCTGAA3650.016.64266817
GAACTCC3800.016.57778421
AGTCACT3400.016.54403328
AGAGCAC3550.016.4777188
TCACTCC3450.016.30479630
CAGTCAC3800.015.98676127
Produced by FastQC (version 0.11.2)