FastQCFastQC Report
Wed 29 Oct 2014
5hr_7_TCCGGAGA-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_7_TCCGGAGA-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3519098
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273540.7773014562254305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1001.3478711E-924.74817813
GCACACG1104.0636223E-922.49834411
ATATCTC6150.022.3306621
CACACGT1201.10812834E-820.62348212
TCGCGCC800.001709056316.8790343
TATCTCG8300.016.8114872
ATCTCGA8950.015.5903183
GTCACTC1451.8105529E-615.51786429
CACGTCT1602.9705188E-715.46761114
ACTCCGG1553.620762E-614.51671132
TATTATG950.00537040414.2139243
ACGTCTG1801.125085E-613.74898915
TCGTATG1656.9202833E-613.63613545
TCCCCCG1000.00755591813.49977543
TCTCGAG11850.012.7243794
AGAGCAC2502.1515007E-812.5990728
AATCTCG2003.6695274E-612.37461841
ATCTCGT2106.3264615E-611.78534942
TGGATCC12400.011.6155154
CTCGAGG13000.011.5987625
Produced by FastQC (version 0.11.2)