FastQCFastQC Report
Wed 29 Oct 2014
5hr_7_TCCGGAGA-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_7_TCCGGAGA-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435764
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110240.4525890028754838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG801.0552321E-725.31142611
CACACGT851.8962419E-723.8225212
ATATCTC3900.023.667421
ACACGTC1051.4475136E-619.28489713
TATCTCG5200.018.1752912
ATCTCGA5600.016.8770563
ACTCCGG1451.8107767E-615.517220532
TGGATCC7800.014.4245384
GTCACTC1251.3819544E-414.39938929
TCTCGAG7000.014.144294
GGATCCG8550.013.4224125
ATCTCGT1709.413203E-613.23527642
CTCGTAT1701.3789933E-411.91174944
ACGTCTG1902.951769E-511.84160215
CTCGAGG8150.011.5962665
CTCCAGT1751.806762E-411.57093824
CACGTCT1751.806762E-411.57093814
AGAGCAC1751.806762E-411.5709388
TCGAGGG8550.011.052396
AATCTCG1903.8703042E-410.65788141
Produced by FastQC (version 0.11.2)