FastQCFastQC Report
Wed 29 Oct 2014
5hr_6_TCCGGAGA-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_6_TCCGGAGA-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888570
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47550.535129477700125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG1300.031.15454931
CTCGTAT1300.029.4237444
GTCACTC1350.028.33397129
CACGTCT1450.027.9300914
GCACACG1400.027.32050711
CACACGT1400.027.32050712
ACTCCGG1500.027.00060832
ACGTCTG1450.026.3784215
TCGTATG1500.025.50057445
ACACGTC1750.023.14207613
CTCCAGT1750.021.85640524
TATCTCG2050.020.8564762
AATCTCG2100.019.28506341
TCTCGTA2000.019.12543143
ATCTCGT2250.018.9993642
ACTCCAG2200.018.4084723
TCCGGAG2251.8189894E-1217.00038134
AGAGCAC2301.8189894E-1216.6298738
GAACTCC2403.6379788E-1215.93696121
GAGCACA2600.015.5763979
Produced by FastQC (version 0.11.2)