FastQCFastQC Report
Wed 29 Oct 2014
5hr_6_TCCGGAGA-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_6_TCCGGAGA-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232417
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99400.3075098293320447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG4200.028.39091511
CACTCCG4400.027.61345731
ACACGTC4400.027.1004213
CACACGT4400.027.1004212
ACTCCGG4600.025.92374632
CTCGTAT4400.025.57038944
TCGTATG4600.024.45825445
CACGTCT5000.024.29833814
ACGTCTG5250.022.71273215
GTCACTC6100.020.2864429
AGAGCAC7050.018.5099458
ATCTCGT6900.017.28329842
CTCCAGT6950.017.15709924
TATCTCG7900.016.5209242
TCTCGTA7100.016.48029543
AATCTCG7150.016.04959141
TCTGAAC8150.014.90695518
GAGCACA8850.014.7447549
CAGTCAC8100.014.72121527
TCACTCC8600.014.65106730
Produced by FastQC (version 0.11.2)