FastQCFastQC Report
Wed 29 Oct 2014
5hr_5_TCCGGAGA-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_5_TCCGGAGA-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899121
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67430.35505899834713006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2200.029.65747611
CACACGT2400.027.18602212
ACACGTC2850.025.26178413
CACGTCT2800.023.30230314
CACTCCG2950.022.11860731
ACTCCGG2800.021.6963932
ACGTCTG3050.021.39227915
TCGTATG3050.020.65515745
CTCGTAT3550.017.74598144
GTCACTC3750.016.7999729
AGAGCAC4050.016.665768
TGCGCGC900.003749908215.0031333
TATCTCG4650.015.0031332
ATCTCGT4850.013.91713542
ATATCTC4900.013.7856491
ACTCCAG5000.013.49926723
CTCCGGA4900.013.31630333
GATCGGA4750.013.2729211
GAGCACA5100.013.2345749
CTCCAGT4950.013.18110124
Produced by FastQC (version 0.11.2)