FastQCFastQC Report
Wed 29 Oct 2014
5hr_5_TCCGGAGA-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_5_TCCGGAGA-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3685882
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73360.19902970306699996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5150.026.64852712
GCACACG5250.025.71239511
ACACGTC5500.024.5436513
CACTCCG5400.023.75018531
ACTCCGG5800.022.50048332
CTCGTAT5950.022.31140344
ACGTCTG6400.021.09248515
TCGTATG6350.020.55134245
CACGTCT6500.020.42157414
GTCACTC6400.020.39023229
TATCTCG8500.017.2083532
TCTCGTA7950.016.69847143
ATCTCGT7900.016.5193442
AATCTCG8050.016.49103741
AGAGCAC8950.016.0888588
CTCCAGT8750.015.42785524
GAACTCC9900.014.09025521
TCACTCC9800.014.00521130
GAGCACA9850.013.9329859
TCCGGAG9750.013.84645134
Produced by FastQC (version 0.11.2)