FastQCFastQC Report
Wed 29 Oct 2014
5hr_4_ATTACTCG-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_4_ATTACTCG-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068042
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53920.5048490602429493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG2400.025.3154242
ACACGTC550.00393799620.45307713
ATATCTC3400.019.1996861
ATCTCGA4050.015.0017323
TCTCGAG4100.014.8173974
CTCGAGG4800.012.6565275
CGAGGGC4900.011.4787677
TCGAGGG5100.011.4697656
GGATCCG5150.011.3594714
GAGGGCG5200.010.8165328
AGGGCGC5500.010.2265399
GGGCGCG5550.010.1344078
GCCGGAT6850.09.19642116
TGGATCC5352.8194336E-108.8327963
ATATGGA5658.913048E-118.7649471
ATCCGGC6750.08.6660446
GGATCCT7300.08.32132119
CGGATCC7051.8189894E-128.297276518
GATCCGG6951.0913936E-118.0937025
CCGGATC7405.456968E-127.90483817
Produced by FastQC (version 0.11.2)