FastQCFastQC Report
Wed 29 Oct 2014
5hr_4_ATTACTCG-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_4_ATTACTCG-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4725989
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135110.2858872502665579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3450.021.52011513
GCACACG3650.020.3409311
TATCTCG12050.019.048642
CACACGT4100.018.65712712
ATATCTC13050.018.1139621
TTACTCG4100.018.11011335
ACGTCTG4350.018.10208115
CACGTCT4500.016.99871614
TCGTATG5250.015.42952945
AGAGCAC5400.014.582858
CTCGTAT5500.013.90995544
TCTCGAG18300.013.403184
ATCTCGA17150.013.2528133
GTCACAT5900.012.58446129
CATTACT7200.012.18774133
ATCTCGT6400.011.953361542
GAACTCC6850.011.8240521
GATCTCG6750.011.66689841
TCGAGGG20950.011.4929694
GAGCACA6950.011.330089
Produced by FastQC (version 0.11.2)