FastQCFastQC Report
Wed 29 Oct 2014
5hr_3_ATTACTCG-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_3_ATTACTCG-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494561
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196140.7862706103398555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG5800.022.8950392
ATATCTC6650.020.316021
GCACACG908.2612005E-619.99899511
ACACGTC1104.643017E-516.36281213
TCGTATG1401.2567962E-616.0706245
TTACTCG1301.1075346E-515.57676335
ATCTCGA8850.015.512983
CTCGTAT1209.763625E-514.99924544
CACACGT1209.763625E-514.99924512
TCTCGAG10950.012.9476674
CACGTCT1506.456365E-411.99939514
CTCGAGG11800.011.4428555
TCGAGGG12050.011.2027566
ATATGGA11650.011.0168631
TGCGCGC1700.001834301910.5913113
GGATCCG11500.010.5672274
TGGATCC10450.010.337933
ACGTCTG1750.002340906510.28519615
CGAGGGC13450.010.036677
GAGGGCG15400.08.911898
Produced by FastQC (version 0.11.2)