FastQCFastQC Report
Wed 29 Oct 2014
5hr_3_ATTACTCG-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_3_ATTACTCG-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4018593
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186740.4646900046857196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC8950.018.3616831
GCACACG1751.4006218E-1017.99878511
TATCTCG10100.016.9333442
TTACTCG2152.588422E-914.65090435
ACACGTC2306.693881E-913.69472813
CACACGT2359.051291E-913.40335112
ATCTCGA13900.012.7945481
CACGTCT2552.8363502E-812.35210714
ACGTCTG3102.919478E-911.61212115
CTCGTAT3002.2291715E-811.25064144
GGATCCG17300.010.7963525
TCGAGGG18450.010.7332534
TCTCGAG17200.010.728294
TGGATCC17450.010.574594
TATAGCG1500.00878382610.49994541
CTCGAGG18150.010.4148533
CGAGGGC20750.09.5435435
TCGTATG3351.2180681E-69.40340445
CATTACT4203.3167453E-89.10698133
GAGGGCG22800.08.6848136
Produced by FastQC (version 0.11.2)