FastQCFastQC Report
Wed 29 Oct 2014
5hr_2_ATTACTCG-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_2_ATTACTCG-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303679
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192470.5825929214067105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG7400.018.8566282
ATATCTC9450.014.7720761
GCACACG2805.857146E-1012.856070511
ATCTCGA12300.012.6306471
GGATCCG16150.011.1481255
TCGTATG2851.3272074E-711.05237945
TCTCGAG15000.010.6529812
ATATGGA17200.010.603231
CACACGT3002.6943235E-710.49912412
ACACGTC3003.0496067E-69.749186513
TGGATCC19200.09.7288454
TCGAGGG16450.09.7135234
CTCGTAT3053.7623286E-69.58994544
CTCGAGG16000.09.5651763
TATGGAT19000.09.4763372
TTACTCG3351.2181536E-69.40319735
ATCCGGC20350.08.6233597
GATCCGG21300.08.5556256
ACGTCTG3451.7925697E-58.47755315
CGAGGGC18650.08.447025
Produced by FastQC (version 0.11.2)