FastQCFastQC Report
Wed 29 Oct 2014
5hr_2_ATTACTCG-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_2_ATTACTCG-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4839654
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166420.34386755747415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG12000.016.5020142
CACACGT3900.013.84541312
GCACACG3950.013.67015511
ATATCTC15550.012.452071
ACACGTC4850.011.59731813
TCGTATG4700.011.48969745
ATCTCGA17700.011.1878073
TTACTCG4850.011.13377135
ACGTCTG5100.011.02882115
ATATGGA22550.010.3838951
GGATCCG23200.010.2813225
TCTCGAG20600.09.3942554
CACGTCT5801.8189894E-129.30984714
TGGATCC26450.09.1881734
CTCGTAT6003.6379788E-129.00026244
CTCGAGG21200.08.9160925
ATCCGGC25750.08.7373967
TCGAGGG22250.08.595496
ACTCGAT6951.8189894E-128.41707737
TATGGAT27900.08.1461552
Produced by FastQC (version 0.11.2)