FastQCFastQC Report
Wed 29 Oct 2014
5hr_1_ATTACTCG-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_1_ATTACTCG-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664842
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28290.16992603502314335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1950.021.92168811
TCGTATG1900.020.13211845
CACACGT2050.019.75484512
ACACGTC1950.019.61414113
TTACTCG2200.018.41013535
CTCGTAT2403.6379788E-1215.93792544
TATCTCG4100.015.916972
ACGTCTG2453.6379788E-1215.61125615
ACATTAC2950.014.49234732
CACGTCT3000.014.24909614
TACTCGA2853.6379788E-1214.2104836
ATATCTC4950.013.6441041
ACTCGAT2855.4569682E-1113.42020337
GATCTCG2907.2759576E-1113.18921641
ATATGGA9050.011.9404751
AGAGCAC4300.011.5108988
ATCTCGA6850.011.1742231
CATTACT3856.184564E-1111.104526533
TCTCGTA3451.3133103E-911.08725343
CGATCTC3703.583409E-1010.945580540
Produced by FastQC (version 0.11.2)