FastQCFastQC Report
Wed 29 Oct 2014
5hr_1_ATTACTCG-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5hr_1_ATTACTCG-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4084429
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43490.10647755169694466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6450.024.76551412
TTACTCG6650.023.68468735
ACGTCTG6750.023.66482515
GCACACG7150.023.28490311
CTCGTAT6900.022.82710644
TCGTATG7000.022.82244945
ACACGTC7150.022.02625813
TACTCGA8250.019.09129336
CACGTCT8900.017.94804214
GATCTCG9650.017.02127641
ACTCGAT9450.016.9053137
TCTCGTA9450.016.42930643
CGATCTC10000.016.20052340
CATTACT10250.015.80519733
TATCTCG8850.015.5097362
ACATTAC10550.014.9292132
ATCTCGT11000.014.318645542
GATCGGA10700.014.307761
ATCTCGA16850.014.0292971
GTCACAT11250.013.80010929
Produced by FastQC (version 0.11.2)