FastQCFastQC Report
Wed 29 Oct 2014
16hr_9_GAGATTCC-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_9_GAGATTCC-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550140
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273341.0718627212623622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA14100.016.2858771
TATCTCG7850.015.4804592
TATGGAT17050.013.8587692
ATCTCGA16950.013.0162681
TCTCGAG18150.011.6549652
ATATCTC11650.011.594591
TCGAGGG19500.011.309264
CTCGAGG19400.010.9043
CGAGGGC21000.010.715775
TGGATCC25200.010.269284
GGATCCG25750.09.87515455
ATGGATC26100.09.2257793
GAGGGCG24850.09.0538046
AGGGCGC26050.08.5503727
GATCCGG30900.08.2276816
GGGCGCG27650.08.1369648
ATCCGGC32800.07.6138927
GCGCCGG33800.06.85611512
GCCGGAT33500.06.850352314
TACGACG3300.00727216856.81805740
Produced by FastQC (version 0.11.2)