FastQCFastQC Report
Wed 29 Oct 2014
16hr_9_GAGATTCC-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_9_GAGATTCC-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025176
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA258180.641412946912135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA22300.016.7579231
TATCTCG10100.016.2642842
TGCGCGC2051.3187673E-915.3676573
TATGGAT26650.014.9454682
CTGCGCG2001.5745172E-814.6267162
ATCTCGA22900.012.1899861
TCGAGGG25500.011.8246234
TCTCGAG24750.011.7286482
CTCGAGG26000.011.251323
TGGATCC39150.010.8055974
GGATCCG39050.010.718025
CGAGGGC28150.010.6315355
ATATCTC16200.010.2833281
ATGGATC40300.09.9391313
GAGGGCG33550.08.9868596
GATCCGG47950.08.72810656
AGGGCGC35350.08.3379917
ATCCGGC50050.08.1817637
GGGCGCG37800.07.79756558
ACGTCTG4701.769271E-67.659045715
Produced by FastQC (version 0.11.2)