FastQCFastQC Report
Wed 29 Oct 2014
16hr_8_GAGATTCC-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_8_GAGATTCC-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959318
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182000.6150065657019624No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.11283005070762926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG10150.019.9523352
ATATGGA16300.019.7491861
CCCCCCC706.9872383E-419.28562745
TATGGAT21250.015.1423872
ATATCTC13000.015.0652611
ATACGAG6300.013.92780314
ATCTCGA20550.013.8025561
TGCGCGC1504.2717464E-513.501083
TATACGA7150.012.90138513
ACGTCTG7700.011.68904934
TCTCGAG24300.011.66762
TCGTATG3702.910383E-1111.55400245
TGGATCC29800.011.2508994
TCGAGGG27600.011.0063154
ATGGATC29850.011.0059053
GCACACG9000.010.75067930
GGATCCG31600.010.5388185
CGAGGGC29250.010.30851655
CTCGAGG28000.010.2865373
CACACGT8650.010.14514931
Produced by FastQC (version 0.11.2)