FastQCFastQC Report
Wed 29 Oct 2014
16hr_8_GAGATTCC-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_8_GAGATTCC-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4249325
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154990.36474028228012684No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT42830.10079247880545733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA23750.019.2410581
CTGCGCG2251.8189894E-1217.0027732
TATCTCG14900.016.613452
TCGTATG5200.016.4426745
GTCGCGC1301.1060807E-515.5794652
TATGGAT30350.015.2743212
TATACGA9400.014.83935513
ATACGAG8700.014.74031614
TGCGCGC3450.014.3499993
ATCTCGA31350.014.0739191
CTCGTAT6600.012.9548344
TCTCGAG35200.012.914042
ATATCTC18850.012.89761
TCGAGGG37900.012.7058114
ACGTCTG11400.012.43448434
TGGGCGC1506.447072E-412.0018173
CGAGGGC41750.011.5341385
CTCGAGG39700.011.3934133
ATGGATC42900.010.9107433
TGGATCC44600.010.8979984
Produced by FastQC (version 0.11.2)