FastQCFastQC Report
Wed 29 Oct 2014
16hr_7_GAGATTCC-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_7_GAGATTCC-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398732
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108820.4536563484374244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG7050.019.7900492
ATATGGA14150.017.0229421
TGCGCGC1257.6243014E-616.2019673
TATGGAT16300.014.9100952
ATATCTC9450.014.293111
ATCTCGA15550.012.5949411
TCTCGAG16550.012.5093322
TCGAGGG17350.012.3213814
CTGCGCG1400.005253406211.25162
CGAGGGC18800.011.2513655
CTCGAGG18500.010.8256393
ATGCCGC1500.00878063610.5001830
ATGGATC24750.010.0012143
GGATCCG24750.010.0012145
TGGATCC25550.09.9522844
GAGGGCG21850.09.67919356
AGGGCGC22300.09.4838747
GGGCGCG23400.08.9419018
GATCCGG28100.08.8874976
ATCCGGC31400.07.953467
Produced by FastQC (version 0.11.2)