FastQCFastQC Report
Wed 29 Oct 2014
16hr_7_GAGATTCC-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_7_GAGATTCC-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2601244
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72140.2773288472746117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG6450.019.1863572
ATATGGA16250.018.7030371
TGCGCGC1257.632412E-616.2002643
TATGGAT19000.016.1055242
GGATCCG24950.012.7156375
TGGATCC25900.012.3361074
ATGGATC25700.012.1694573
ATCTCGA14200.011.5735421
TCTCGAG15450.011.068142
TCGAGGG16500.011.0456344
GATCCGG29150.010.7287496
ATATCTC11550.010.5255191
CTCGAGG17800.09.9861173
CGAGGGC19000.09.7106845
ATCCGGC32250.09.6276927
GCACACG2350.00270843138.61666411
AGGGCGC21550.08.4568897
GAGGGCG21850.08.4437486
TCCGGCG39500.08.2023498
GGGCGCG22750.08.0108118
Produced by FastQC (version 0.11.2)