FastQCFastQC Report
Wed 29 Oct 2014
16hr_6_CGCTCATT-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_6_CGCTCATT-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981283
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG50690.5165686147625099TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5700.039.4730645
CACACGT5850.038.8435612
CTCGTAT5900.038.13498744
ACCGCTC5900.038.13498732
ACGTCTG6150.037.3145815
ACACGTC6200.037.01365713
CGCTCAT6150.036.95063434
CACGTCT6250.036.71754514
GCACACG6250.036.3575711
CACCGCT6350.035.43250731
TCTCGTA6400.035.15569343
CTCATTA6700.033.5815636
TGCGCGC551.12506314E-732.7350923
TCACCGC6950.032.37358530
ACTCCAG7300.031.12805723
AGTCACC7250.031.0339928
GTCACCG7300.030.8214329
GCTCATT7400.030.40492435
ATCTCGT7500.030.2995242
GATCGGA6650.030.1307281
Produced by FastQC (version 0.11.2)