FastQCFastQC Report
Wed 29 Oct 2014
16hr_6_CGCTCATT-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_6_CGCTCATT-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705279
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG157010.5803837607877043TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC20100.039.8501732
TCGTATG20300.039.34745445
CTCGTAT20950.038.0199544
CACACGT21200.037.99403812
ACGTCTG21300.037.71002615
GCACACG21600.037.2904411
CGCTCAT21650.036.9978434
ACACGTC22050.036.73348613
CACCGCT22000.036.30629731
CTCATTA22500.036.0001536
GTCACCG22100.035.938429
TCACCGC22250.035.7972430
CACGTCT22650.035.56174514
TCTCGTA22650.035.46437543
AGTCACC23050.034.65307228
GCTCATT23850.034.15108535
ATCTCGT23350.034.11211842
CATTATC24900.032.4404938
CAGTCAC24550.032.35187527
TCATTAT25450.031.91565137
Produced by FastQC (version 0.11.2)