FastQCFastQC Report
Wed 29 Oct 2014
16hr_5_CGCTCATT-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_5_CGCTCATT-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281454
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41240.3218219304009352No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.11206020660905502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC751.6944068E-624.0056823
TATCTCG3400.020.5195642
ATATGGA7200.017.8229791
TATGGAT9400.013.8862662
ATCTCGA7600.013.3302061
TCTCGAG8900.012.1377052
ATATCTC5700.011.84907151
TCGAGGG10200.011.6934884
GGATCCG13700.010.6772944
CTCGAGG10600.010.6157213
CGAGGGC11300.010.35602955
TGGATCC12950.010.2529924
ATGGATC12800.010.1977262
AGGTGAG2504.7349223E-48.99967241
GATCCGG16550.08.9780871
GAGGGCG13500.08.8330116
AGGGCGC14550.08.1955787
ATCCGGC20250.07.55734442
GGGCGCG15650.07.47576578
TCCGGCG22450.07.0172523
Produced by FastQC (version 0.11.2)