FastQCFastQC Report
Wed 29 Oct 2014
16hr_5_CGCTCATT-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_5_CGCTCATT-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545746
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53100.20858326007386438No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.11340487228498052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG1004.5474735E-1127.0026172
TGCGCGC1252.910383E-1123.4022663
ATATGGA14800.021.4481
TATGGAT18450.018.050532
TATCTCG8750.018.0017432
ATATCTC10400.015.1528421
ATCTCGA16750.014.9189271
TGGCGCG1150.001197330913.7034411
TCTCGAG20100.013.4341362
TCGAGGG22350.012.9878354
TGGATCC28400.012.281124
GGATCCG27750.012.244435
ATGGATC28050.011.953033
CGAGGGC25250.011.5852815
CTCGAGG24050.011.5083923
GATCCGG33350.010.2552626
GAGGGCG30300.09.5795726
AGGGCGC30600.09.4113837
GGGCGCG32000.08.9996348
ATCCGGC38750.08.7093247
Produced by FastQC (version 0.11.2)