FastQCFastQC Report
Wed 29 Oct 2014
16hr_3_CGCTCATT-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_3_CGCTCATT-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022698
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190931.8669245466403572No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT18510.1809918470555335No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.12369242924108582No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.11655444715839866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTC300.005147598729.9992828
ATATGGA6100.027.6768071
TCGTATG1600.022.50056345
CGCTCAT1653.6379788E-1220.45505734
TATGGAT8450.019.9748212
GTCACCG1703.6379788E-1219.85343729
ACCGCTC1950.019.61587332
ATATCTC3800.019.5485631
CACCGCT2150.018.8376831
CTCGTAT1851.6370905E-1118.24369844
CCGAGGG750.001108831318.003093
ATCTCGA7050.017.5613571
TATCTCG4700.016.2801882
CACACGT2101.07320375E-1016.0702612
GCACACG2505.456968E-1215.29888611
ACGTCTG2253.0559022E-1014.99890715
ATGGATC11600.014.743913
CTCATTA2304.2382453E-1014.67356336
TCACCGC2509.640644E-1114.40035930
TGCGCGC1301.9229975E-413.8485323
Produced by FastQC (version 0.11.2)