FastQCFastQC Report
Wed 29 Oct 2014
16hr_3_CGCTCATT-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_3_CGCTCATT-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584679
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA303781.1753103576885175No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.18017711290260802No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.12500585179049312No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27840.10771163459756511No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.10357185553796042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA16200.027.6535361
CTCGTAT5000.024.75113944
TCGTATG5000.024.75113945
CGCTCAT5100.023.82416534
ACCGCTC5900.021.73786732
CACCGCT5850.021.15431
CTCATTA6100.021.0251536
TATGGAT22000.020.9684962
CACACGT6800.018.85897312
GCACACG6700.018.80465111
ACGTCTG6800.018.52811415
TCACCGC7000.018.32156430
GTCGCGC750.001109813918.0022222
GCTCATT6850.017.737735
CATTATC7300.017.26073538
ACACGTC7600.017.1698513
ATATCTC11450.017.105131
GTCACCG7600.016.282729
ATCTCGA18300.016.1150931
TATCTCG13150.015.401142
Produced by FastQC (version 0.11.2)