FastQCFastQC Report
Wed 29 Oct 2014
16hr_20_TCCGCGAA-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_20_TCCGCGAA-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6343647
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78426712.36303028841296No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288330.4545177245833509No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279100.43996773464853894No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT254610.4013621817229111No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT192450.3033743838520649No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187200.29509838741027045No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186740.2943732524839418No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173540.27356503285885864No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144050.22707757856009328No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140200.22100851450277734No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT137990.21752471409585053No Hit
CGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128410.20242299106491896No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125510.1978514882684992No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT94300.1486526598973745No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85380.13459134784769708No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77390.12199606945342324No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAAA74720.11778713412016778No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAA67000.10561747840004337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA73550.035.718481
ATATCTC45200.031.7253021
TATCTCG57550.024.9859542
TATGGAT108050.024.9084552
TGGGCGC3950.022.2181513
GTGGGCG4650.020.3252892
GTCGCGC2000.020.2526992
ATTCGGC3700.020.077841
ATGGATC135900.019.9364323
TGCGCGC4700.019.6302763
AATAAAA59350.019.3064231
ATAAAAA61650.019.2361412
TCCGCGA2900.017.84481634
TGGATCC162500.017.4063854
CGCGGTA3000.017.24985132
CTGCGCG3400.017.2081762
ATCTCGA105300.017.1455251
AACCGGC9600.017.1116582
TCTCGAG121000.015.677712
GGATCCG182450.015.5400865
Produced by FastQC (version 0.11.2)