FastQCFastQC Report
Wed 29 Oct 2014
16hr_1_CGCTCATT-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_1_CGCTCATT-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627632
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82261.3106406301781937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG702.8827344E-828.927711
ACACGTC755.6323188E-826.99918713
CACACGT755.6323188E-826.99918712
CACGTCT856.4719643E-926.46979114
ACGTCTG801.05250365E-725.31173715
CTCGTAT851.8913488E-723.82281344
ATATGGA3400.018.5362381
GCTCATT1406.4643245E-817.6780435
CTCATTA1305.774091E-717.3071736
TCTCGTA1401.2528708E-616.07094443
CGCTCAT1301.10510955E-515.57645334
TCGTATG1301.10510955E-515.57645345
TATGGAT4000.015.1906732
ACCGCTC1351.5797297E-514.99954832
CACCGCT1553.6119618E-614.51569231
CAGTCAC1605.0189865E-614.06207727
ATCTCGT1453.100819E-513.96509742
ATCTCGA3750.013.8050831
ATATCTC2451.0841177E-913.7805841
TCACCGC1656.899578E-613.63595330
Produced by FastQC (version 0.11.2)