FastQCFastQC Report
Wed 29 Oct 2014
16hr_1_CGCTCATT-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_1_CGCTCATT-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401848
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185710.7731963055114229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3000.022.49876212
TGCGCGC1202.4976362E-718.751313
GCACACG3650.018.49213411
ACGTCTG4000.017.99900815
ATATGGA11900.017.5944211
ACACGTC4350.017.06802613
TCGTATG4050.016.66783145
CTCGTAT4350.015.51832544
CGCTCAT4550.015.33010234
ACCGCTC4600.015.16315632
CACGTCT4750.015.15706114
TATCTCG9050.013.9236252
TATGGAT16350.013.4871812
TCTCGTA5100.013.23621843
CTCATTA5100.013.23566736
ATATCTC9950.012.6707971
CACCGCT5300.012.31141231
CAGTCAC6000.011.6243627
GTCACCG6200.011.24961629
TCACCGC6450.011.16287330
Produced by FastQC (version 0.11.2)