FastQCFastQC Report
Wed 29 Oct 2014
16hr_19_CGGCTATG-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_19_CGGCTATG-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614211
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA706941.5320929190277601No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT66550.1442283415301121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA29900.024.2434641
ATATCTC19450.020.0233351
TGCGCGC2600.019.9077973
TATCTCG20000.019.4665742
CTGCGCG1608.1672624E-1018.2850762
GTCGCGC1507.2632247E-918.0037672
TATGGAT41600.017.5818042
GGGGCGC901.8642301E-417.5036622
CGGGGCG800.001705913616.8838391
GTGGGCG2101.07320375E-1016.0747932
ATCTCGA37850.015.9991281
TCGCGCC1602.9619514E-715.4718193
TCTCGAG44550.013.841282
TGGGCGC2451.0950316E-913.7782433
TGGCGCG2602.6630005E-912.9833463
ATGGATC59100.012.6421033
TGGATCC62250.012.3995134
ATTCGGC2603.6876372E-812.1217311
TCGAGGG54200.012.0406124
CTCGAGG52550.011.9909623
Produced by FastQC (version 0.11.2)