FastQCFastQC Report
Wed 29 Oct 2014
16hr_19_CGGCTATG-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_19_CGGCTATG-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189854
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA301080.943867650368951No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT43840.13743575724782386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA20000.021.4983081
TGCGCGC2050.019.7579753
GTCGCGC1555.347829E-1018.8727632
CTGCGCG1802.0736479E-1017.5016652
TCGCGCC1459.67284E-817.070593
ATCTCGA23950.016.7307471
ATATCTC12400.015.79422951
TATCTCG11950.015.2587171
TATGGAT29050.015.1047072
TCTCGAG28650.014.6872612
TCGAGGG35200.012.7849064
CTCGAGG33000.012.3420833
GGATCCG42150.011.4776014
TGGATCC40500.011.3899723
ATGGATC40700.011.2234273
CGAGGGC41300.011.0055455
CCCGGCG1500.00877662910.50099853
TCGGCGC3003.0425454E-69.7509273
GAGGGCG48500.09.3709976
CCGAGGG3155.633625E-69.2903851
Produced by FastQC (version 0.11.2)