FastQCFastQC Report
Wed 29 Oct 2014
16hr_18_GAATTCGT-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_18_GAATTCGT-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263915
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570112.9827799271074857No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT70250.13345580238282723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA29800.021.7577761
TGCGCGC3100.020.3275993
TATGGAT38100.017.130212
TATCTCG20700.016.4170952
ATCTCGA40950.014.6789711
TGGGCGC1705.8827936E-714.5624173
TCTCGAG44350.013.9549662
TCGAGGG50250.012.9881554
ATATCTC25350.012.9662131
TGGATCC55850.012.3305894
ATGGATC55500.012.0435123
CTCGAGG50550.012.020773
CTGCGCG3408.185452E-1111.9147042
CCCCCCC2901.3526005E-811.63792944
GGATCCG58600.011.5215055
CGAGGGC58200.011.3300085
ATAAAAA23500.010.34297852
ATTCGGC4355.638867E-109.8333751
GTGGGCG2555.3965396E-59.7082782
GAGGGCG69600.09.3742866
Produced by FastQC (version 0.11.2)