FastQCFastQC Report
Wed 29 Oct 2014
16hr_18_GAATTCGT-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_18_GAATTCGT-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841926
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA698011.8168231246515418No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.13006497262050337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA23000.020.4591061
GTCGCGC1555.347829E-1018.8738022
TAGGGCG800.00170431116.8861081
TATGGAT29000.016.1403542
ATCTCGA27650.015.715581
TGCGCGC2302.5465852E-1115.6545233
CTGCGCG1502.5732897E-615.0022532
TCGCGCC2101.8517312E-915.0022523
TCTCGAG29150.014.976522
TCGAGGG32950.014.2051014
TATCTCG13000.013.3289242
CTCGAGG33600.013.2609193
CGAGGGC37700.012.59445
ATGGATC41750.011.5346653
TGGATCC42200.011.5180174
ATATCTC15650.011.5091671
GGATCCG43300.011.2254115
GAGGGCG45150.010.4653256
AGGGCGC45850.010.2559417
GGGCGCG49800.09.4424688
Produced by FastQC (version 0.11.2)