FastQCFastQC Report
Wed 29 Oct 2014
16hr_17_GAATTCGT-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_17_GAATTCGT-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325252
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA513591.1874221432647163No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.11553084074638889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCG1302.7721399E-819.041712
TATCTCG14650.016.897082
ATATGGA23150.016.7261051
TGCGCGC2301.8189894E-1216.633083
TGGGCGC1951.1272277E-815.0023853
ATATCTC18750.013.44726851
TATACGA11000.012.88543413
TATGGAT32600.012.7062551
ACGTCTG12250.012.30651534
ATCTCGA29100.012.2231031
CCCCCCC1852.245852E-512.1624145
ATACGAG11800.011.8211814
TCTCGAG31450.011.7349112
GCACACG13350.011.62958430
TGGCGCG4900.011.4814193
CACACGT13200.011.42081831
TCGAGGG35600.010.8721394
ACACGTC13950.010.80679632
GTGGGCG2503.6891415E-610.8018422
GGATCCG44700.010.6221274
Produced by FastQC (version 0.11.2)