FastQCFastQC Report
Wed 29 Oct 2014
16hr_17_GAATTCGT-CCTATCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_17_GAATTCGT-CCTATCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5742301
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA411080.7158802716889971No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT61540.10716958236776511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA33150.018.133391
GTCGCGC1406.47633E-817.6816582
TATCTCG18400.016.1441212
TGCGCGC3400.015.8848493
TATGGAT41250.014.5116232
ATCTCGA38450.013.1160481
TCTCGAG43250.012.4356972
ATATCTC24350.012.3895951
TATACGA13650.012.19706413
CTGCGCG2603.7063728E-812.1174992
TCGAGGG47400.012.0108584
ATACGAG14000.011.73143214
TGGGCGC2502.9116745E-711.70183853
GTGGGCG3002.2262611E-811.2519652
CGAGGGC52500.010.8012265
TGGATCC57600.010.7434084
ATGGATC56050.010.7197923
CTCGAGG50650.010.6630773
CACACGT16100.010.62093731
GGATCCG58650.010.4743365
Produced by FastQC (version 0.11.2)