FastQCFastQC Report
Wed 29 Oct 2014
16hr_16_GAATTCGT-ATAGAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_16_GAATTCGT-ATAGAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799128
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2236215.88611386612928No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT51970.13679454864379403No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41520.10928823666904615No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40420.10639283540854638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG5800.028.7049811
ACACGTC6200.026.4901713
CACACGT6300.026.0696912
ACGTCTG7200.023.74841715
CACGTCT7500.022.49849914
CTCGTAT7600.022.2042244
TCGTATG7500.022.20027245
TGCGCGC2650.022.078943
ATATGGA21700.021.6813341
TTCGTAT7850.021.20933537
TCGTATC8200.020.02994338
ATTCGTA8100.019.99945636
AGTCACG8900.018.4557728
AATAAAA18800.017.9610941
CACGAAT9250.017.75767531
ATATCTC18150.017.7361281
TCTCGTA10000.017.32521243
GTCACGA10250.017.12283729
ATAAAAA20300.016.7390822
CGAATTC9900.016.59176833
Produced by FastQC (version 0.11.2)