FastQCFastQC Report
Wed 29 Oct 2014
16hr_16_GAATTCGT-ATAGAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_16_GAATTCGT-ATAGAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412265
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530453.4686266577370124No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT56630.12834677880861645No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.10284967018073483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6900.024.12908612
GCACACG7100.023.44939211
ATTCGTA7400.022.50027336
TCGTATC7900.020.79162638
ATATGGA24300.020.5662921
ACGTCTG8250.020.45340315
TCGTATG8650.020.28926345
ACACGTC8550.020.26202613
TTCGTAT8450.019.97065537
CTCGTAT8850.019.57672944
TGCGCGC2700.019.1679863
CACGTCT9950.017.86332514
AGTCACG9800.017.67838328
CGAATTC9750.017.30790133
GTCACGA10100.017.15328429
CACGAAT10150.016.84749431
ATCTCGA32550.014.8003521
TATGGAT34450.014.7615382
CTGCGCG2601.0913936E-1114.712552
TATCTCG19400.014.6144072
Produced by FastQC (version 0.11.2)