FastQCFastQC Report
Wed 29 Oct 2014
16hr_15_GAATTCGT-TATAGCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_15_GAATTCGT-TATAGCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867701
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA367930.9512886337387507No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT41540.10740230436634063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA21100.020.6986871
TATCTCG14200.017.9088442
GTCGCGC1104.633395E-516.3672142
ATATCTC16850.015.6318181
TATGGAT28700.015.5260432
TCGCGCC1352.6498668E-413.3360773
TATACGA7700.012.56427113
ATCTCGA24400.012.5479451
TGGATCC40000.011.8708854
GGATCCG40200.011.5879055
ATGGATC39650.011.5219163
ATACGAG7750.011.32198214
TCTCGAG28450.011.1535812
TACGAGA8650.010.92427815
TCGAGGG31200.010.7471254
CTCGAGG31350.010.1934853
CGAGGGC35600.09.4820425
GATCCGG52250.08.9564656
GAGGGCG40450.08.3988146
AGGGCGC41250.08.12684257
Produced by FastQC (version 0.11.2)