FastQCFastQC Report
Wed 29 Oct 2014
16hr_15_GAATTCGT-TATAGCCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_15_GAATTCGT-TATAGCCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4124743
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241400.5852485839723832No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.10223667268481938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC2300.019.5666033
ATATGGA23050.018.361171
TATCTCG14050.016.6561852
ATATCTC15400.015.05670451
TATGGAT30000.014.3261892
ATCTCGA26350.014.0112421
ATACGAG6350.013.81803514
CTGCGCG2602.6666385E-912.9818472
TCTCGAG29850.012.6643682
TCGAGGG32550.012.1666134
TATACGA7250.011.79236713
CTCGAGG32500.011.5623573
ATTCGTA4101.8189894E-1211.52436836
GGATCCG38750.011.4393825
TCGTATG4550.011.37415745
CGAGGGC36500.011.2198375
TTCGTAT4152.1827873E-1110.84335337
TGGATCC42950.010.6874734
ATGGATC44200.010.1308083
GTACGTC1850.00371104859.7291219
Produced by FastQC (version 0.11.2)