FastQCFastQC Report
Wed 29 Oct 2014
16hr_14_ATTCAGAA-GGCTCTGA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_14_ATTCAGAA-GGCTCTGA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4991737
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1683213.3719925548962215No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT121260.24292145199156126No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104970.21028752115746482No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78240.15673902691588118No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT77380.15501617973863607No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64870.1299547632417333No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT62310.12482628792342225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGGA33050.027.588851
ATATCTC26000.023.6395571
TATCTCG26250.023.31862
TGCGCGC2850.018.9506843
TATGGAT47950.018.9138222
ATCTCGA46700.015.9091541
GTCGCGC1156.7790505E-515.6550682
GGTCGCG1451.8000956E-615.5268021
ATGGATC64500.014.200163
GTGGGCG2408.039933E-1014.0651032
TGGGCGC2451.0950316E-913.7779213
TGGATCC69600.013.5472224
TCTCGAG58350.013.4215052
GGATCCG75050.012.6234145
TCGAGGG66500.012.4190924
CTCGAGG64300.012.3194193
TAGGGCG1300.003019973612.1175043
ATAAAAA21950.011.4827622
GATCCGG99750.010.9917311
AATAAAA22450.010.6301541
Produced by FastQC (version 0.11.2)