FastQCFastQC Report
Wed 29 Oct 2014
16hr_14_ATTCAGAA-GGCTCTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_14_ATTCAGAA-GGCTCTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881747
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA854962.202513455925901No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT94950.24460635894096136No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87180.22458959844626658No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61010.1571715003579574No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60800.15663050683107374No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49620.12782904192364933No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT47480.12231606026873983No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.10894579167575835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC1105.456968E-1226.5931342
ATATGGA28800.026.4224131
TATCTCG17300.021.59142
ATTCGGC1900.021.3288361
ATATCTC18900.019.7740021
TATGGAT39850.019.3115252
ATCTCGA32150.015.6160841
ATGGATC51600.015.0448633
TGCGCGC2405.0931703E-1115.0012533
TGGATCC55700.014.4624234
GGATCCG59350.013.7246435
TAGGGCG1504.251112E-513.5082651
ACCCGGC1851.5288861E-613.3794972
TCGTATG4050.013.33324545
TCTCGAG40400.012.9218712
CACACGT3900.012.69173412
CTGCGCG2301.3558983E-611.7401112
TCGAGGG49600.011.6137244
CTCGAGG46750.011.45550253
GATCCGG81300.010.6338111
Produced by FastQC (version 0.11.2)