FastQCFastQC Report
Wed 29 Oct 2014
16hr_13_ATTCAGAA-CCTATCCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr_13_ATTCAGAA-CCTATCCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524514
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA399010.8818847725965705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGC1650.021.8232043
ATATGGA22000.016.5749261
TATCTCG12750.015.3564752
TATGGAT27000.013.0029932
ATATCTC16100.012.30314351
ATCTCGA22100.010.9999511
TCGCGCC1700.00183572110.59067253
GGATCCG36050.010.5499295
TGGATCC36700.010.4243964
TCTCGAG25800.010.0313782
ACCCGGC2302.0487022E-49.7848612
TCGAGGG27700.09.5867254
CTGCGCG3206.906297E-69.1427292
CCTAGGC2050.0086025588.7840621
GATCCGG44550.08.5852736
CGAGGGC31800.08.4922395
ATGGATC43600.08.465253
CTCGAGG30150.08.3601333
ATCCGGC48400.07.90235337
CCCGGCG3451.6443746E-47.82788853
Produced by FastQC (version 0.11.2)